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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which results in chromosome instability, and so might lead to tumorigenesis. This protein was discovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a crucial function in selling mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been explained. Two additional splice variants have been described but comprehensive nucleotide sequence information and facts is not but accessible.
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Blocking peptides are peptides that bind exclusively to your focus on antibody and block antibody binding. These peptide normally includes the epitope acknowledged with the antibody.
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This tutorial outlines further more possible optimization of cellular and immunoassay parameters to make certain the best possible outcomes are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to be sure the absolute best success are...
Antibodies bound to the blocking peptide not bind for the epitope about the focus on protein. By evaluating the staining through the blocked antibody as opposed to the antibody on your own, one can see which staining is specific.
Target Information and facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterized by a extraordinary periodicity in protein abundance with the mobile cycle. Cyclins operate as regulators of CDK kinases. Different cyclins show distinct expression and degradation patterns which add to your temporal coordination of every mitotic party. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is particularly degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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